Noha Bassiouny Hassan
Ain Shams University Hospital, Egypt
Title: Role of immune staining in detecting extra-pattern and subtle lymphomatous infiltration in bone marrow biopsies of NHL patients
Biography
Biography: Noha Bassiouny Hassan
Abstract
Introduction & Aim: Immunohistochemistry (IHC) enables the examination of a greater number of trephine biopsy levels and is helpful in determining additional scattered malignant cells. The aim of this study is to detect extrapattern and subtle lymphomatous infiltration in bone marrow biopsies using CD20 and CD3 immunostaining.
Patients & Methods: This study was conducted on 100 newly diagnosed Non Hodgkin Lymphoma (NHL) patients. Their bone marrow trephine biopsies were assessed on routine histology [Hematoxylin and Eosin (H & E)] and were
further subjected to IHC using CD20 and CD3.
Results: Pattern of involvement by H&E was highlighted by IHC. It showed additional interstitial pattern in nine cases, parasinusoidal streaks in one case and highlighted a patchy pattern in another case with interstitial involvement
on H&E. IHC also detected subtle infiltrations on additional 5.5% cases compared with histology alone. It helped in differentiating reactive (12 cases) and malignant lymphoid infiltration (33 cases).
Conclusion: CD20 and CD3 immunostaining performed routinely on bone marrow trephine biopsies has the ability to reveal extra-pattern of infiltration and improve detection of subtle lymphoid involvement. A combined procedure identifying several distinctive features in particular histotopography and IHC, provides a promising way of discriminating reactive from neoplastic lymphoid infiltrates in bone marrow trephine biopsies.